@kennethamy,
We can only be certain that something does not exist in the universe if its existence is contradictory to the physical laws of the universe, a rock so big that it cannot be moved or cannot move, for example (?). Even then, the idea of such a thing will exist in the mind. Therefore, we cannot say that unicorns do not exist because their existence is not contradictory to the known physical laws of the universe. Unicorns may certainly exist in the universe somewhere, if not on earth. We may not even say that a unicorn has not existed on the earth at some time in the past, and certainly there is more evidence in support of their existence than in rejection of their existence.
To reject the existence of an entity, we must first have the idea of that entity in our minds, must conceive it clearly with all its properties and attributes. It must exist in the mind before it's existence in reality (in the universe) can be rejected. Therefore, in the broadest sense of the question, it is impossible for anything not to exist, if its existence may be questioned.
As for the existence of entities in the universe (or multiverse?), we must be specific with regard to the space-time location of the entity in question, unless its existence contradicts the physical laws of the universe. Otherwise, almost anything can exist somewhere as far as we may know.
Samm